Journal: Drug Design, Development and Therapy
Article Title: Cytoprotective effect of selective small-molecule caspase inhibitors against staurosporine-induced apoptosis
doi: 10.2147/DDDT.S60283
Figure Lengend Snippet: Hierarchical clustering of 200 upregulated and downregulated genes from NIH3T3 samples treated with DMSO, RBC1023, STAU, or RBC1023 plus STAU. Notes: NIH3T3 cells were treated with DMSO, 20 μM of RBC1023 (c23), 1 μM of STAU, or 20 μM of RBC1023 (c23) plus 1 μM of STAU for 20 hours. Total RNA was isolated and converted to complementaryDNA by reverse transcriptase reaction. The cDNA products were fragmented and hybridized to Affymetrix GeneChip Mouse Gene 1.0ST (Affymetrix Inc., Santa Clara, CA, USA). Clustering of 200 upregulated and downregulated genes that exhibit significant differences in all groups was determined by one-way analysis of variance. The clustering was based on fold changes within each paired data set. The color scale of the log2 ratios is shown at the bottom. Abbreviations: DMSO, dimethyl sulfoxide; STAU, staurosporine.
Article Snippet: Labeled cDNA was added to Affymetrix hybridization cocktails, heated at 99°C for 5 minutes, and hybridized for 16 hours at 45°C to GeneChip Mouse Gene 1.0ST Arrays (Affymetrix Inc.).
Techniques: Isolation